FSD1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FSD1 Polyclonal Antibody, Unconjugated (bs-13220R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FSD1 Polyclonal Antibody, Unconjugated (bs-13220R) at 1:400 overnight at 4\u00b0C, DAB staining.

Lane 1: Mouse Brain lysates; Lane 2: Mouse Cerebellum lysates probed with FSD1 Polyclonal Antibody, Unconjugated (bs-13220R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-13220R
Product Name	FSD1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FSD1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79187
Subcellular location	Cytoplasm, Nucleus
Synonyms	Fibronectin type 3 and SPRY spla, ryanodine domain containing with coiled-coil mot 1; Fibronectin type 3 and SPRY domain containing 1; Fibronectin type III and SPRY domain containing 1; Fibronectin type III and SPRY domain-containing protein 1; Fibronectin type III and SPRY domains containing protein 1; FSD1; FSD1_HUMAN; GLFND; MGC3213; MGC58341; Microtubule-associated protein GLFND; MID1-related protein 1; midline 1-related protein 1; MIR1; VLP27.
Background	May be involved in microtubule organization and stabilization.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200