GFM2 Polyclonal Antibody

$HepG2 (Human cell line) lysates probed with GFM2 Polyclonal Antibody, unconjugated (bs-13339R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

HepG2 (Human cell line) lysates probed with GFM2 Polyclonal Antibody, unconjugated (bs-13339R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Sheep
Pig

Overview
Catalog #	bs-13339R
Product Name	GFM2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GFM2
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84340
Swiss Prot	Q969S9
Subcellular location	Cytoplasm
Synonyms	EF-G2mt; EFG2; EFG2mt; elongation factor G 2, mitochondrial; Elongation factor G2; G elongation factor mitochondrial 2; GFM2; hEFG2; mEFG 2; mEFG2; Mitochondrial elongation factor G2; mitochondrial ribosome recycling factor 2; MRRF2; MST027; MSTP027; OTTHUMP00000222951; OTTHUMP00000222952; ribosome-releasing factor 2, mitochondrial; RRF2; RRF2mt.
Background	GFM2 is a mitochondrial translation elongation factor. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200