SCIN Polyclonal Antibody

Lane 1: Mouse Kidney lysates probed with SCIN Polyclonal Antibody, Unconjugated (bs-17286R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCIN Polyclonal Antibody, Unconjugated (bs-17286R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SCIN Polyclonal Antibody, Unconjugated (bs-17286R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-17286R
Product Name	SCIN Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SCIN
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	Q9Y6U3
Swiss Prot	85477
Synonyms	Adseverin; KIAA1905; ADSV_HUMAN; Scinderin.
Background	SCIN (Scinderin) is a Ca(2+) dependent actin filament severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro SCIN also has barbed end capping and nucleating activities in the presence of Ca(2+).
Application Dilution
WB
IHC-P	1:200-400
IHC-F
IF