SLC10A1 Polyclonal Antibody

$Mouse kidney cells probed with Rabbit Anti-NTCP\/SLC10A1 Polyclonal Antibody, FITC Conjugated (bs-1958R-FITC) (green) at 1:50 for 40 minutes compared to control cells (red).$

Mouse kidney cells probed with Rabbit Anti-NTCP\/SLC10A1 Polyclonal Antibody, FITC Conjugated (bs-1958R-FITC) (green) at 1:50 for 40 minutes compared to control cells (red).

Human HepG2 and A431 lysates probed with Rabbit Anti-SLC10A1 Polyclonal Antibody, Unconjugated (bs-1958R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Mouse liver lysates probed with Rabbit Anti-SLC10A1 Polyclonal Antibody, Unconjugated (bs-1958R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$HEK 293T cells probed with NTCP\/SLC10A1 Polyclonal Antibody, Unconjugated (bs-1958R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

HEK 293T cells probed with NTCP\/SLC10A1 Polyclonal Antibody, Unconjugated (bs-1958R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Lane 1: Human HL60 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U-2 OS cell lysates probed with SLC10A1 Polyclonal Antibody, Unconjugated (bs-1958R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HL60 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U-2 OS cell lysates probed with SLC10A1 Polyclonal Antibody, Unconjugated (bs-1958R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse
Rat

Overview
Catalog #	bs-1958R
Product Name	SLC10A1 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NTCP
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6554
Swiss Prot	Q14973
Subcellular location	Cell membrane
Synonyms	NTCP; Sodium/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; SLC10A1; GIG29
Background	The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000