PACE4 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: Rat Testis tissue lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human SH-SY5Y cell lysates probed with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Testis tissue lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human SH-SY5Y cell lysates probed with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-19997R
Product Name	PACE4 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PACE4
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5046
Swiss Prot	P29122
Synonyms	Paired basic amino acid cleaving enzyme 4; paired basic amino acid cleaving system 4; PCSK6; PCSK6_HUMAN; Proprotein convertase subtilisin/kexin type 6; SPC4; Subtilisin like protease; Subtilisin-like proprotein convertase 4; subtilisin/kexin like protease PACE4; Subtilisin/kexin-like protease PACE4.
Background	Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Application Dilution
WB
ELISA