GLRB Polyclonal Antibody

Lane 1: Mouse Cerebrum; Lane 2: Mouse Cerebellum lysates probed with GLRB Polyclonal Antibody, Unconjugated (bs-20450R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.

U251 cell lysates probed with GLRB Polyclonal Antibody, Unconjugated (bs-20450R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Spinal cord tissue lysates probed with GLRB Polyclonal Antibody, Unconjugated (bs-20450R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Spinal cord tissue lysates probed with GLRB Polyclonal Antibody, Unconjugated (bs-20450R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-20450R
Product Name	GLRB Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GLRB
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2743
Swiss Prot	P48167
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Glycine receptor subunit beta; GLRB; Glycine receptor 58 kDa subunit
Background	Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:8717357, PubMed:15302677, PubMed:16144831, PubMed:22715885, PubMed:25445488, PubMed:11929858, PubMed:23238346). Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1 (PubMed:8717357). Plays an important role in the down-regulation of neuronal excitability (PubMed:11929858, PubMed:23238346). Contributes to the generation of inhibitory postsynaptic currents (PubMed:25445488).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200