RYBP Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RYBP Polyclonal Antibody, Unconjugated (bs-2053R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RYBP Polyclonal Antibody, Unconjugated (bs-2053R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RYBP Polyclonal Antibody, Unconjugated (bs-2053R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RYBP Polyclonal Antibody, Unconjugated (bs-2053R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Horse
Chicken

Overview
Catalog #	bs-2053R
Product Name	RYBP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RYBP
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23429
Subcellular location	Cytoplasm, Nucleus
Synonyms	AAP 1; AAP1; APAP 1; APAP-1; APAP1; Apoptin associating protein 1; Apoptin-associating protein 1; cb337; Death Effector Domain Associated Factor; Death effector domain-associated factor; Death effector domain-associated factor B; DED associated factor; DED-associated factor; DED-associated factor B; DEDAF; RING1 and YY1 binding protein a; RING1 and YY1 binding protein; RING1 and YY1-binding protein; RING1 and YY1-binding protein B; Ring1 interactor RYBP; RYBP; RYBP_HUMAN; rybpa; YEAF 1; YEAF1; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; YY1- and E4TF1/GABP-associated factor 1.
Background	May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200