CPSF4 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 27kD. Observed band size: 23kD, 27kD.$

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti CPSF4\/CPSF30 Polyclonal Antibody, Unconjugated (bs-2253R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 27kD. Observed band size: 23kD, 27kD.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2253R
Product Name	CPSF4 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CPSF4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10898
Swiss Prot	O95639
Subcellular location	Nucleus
Synonyms	NAR; NEB1; CPSF30; Cleavage and polyadenylation specificity factor subunit 4; Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; Neb-1; No arches homolog; CPSF4
Background	Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500