EML4 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EML4-ALK fusion protein(EML4) Polyclonal Antibody, Unconjugated (bs-24711R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EML4-ALK fusion protein(EML4) Polyclonal Antibody, Unconjugated (bs-24711R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-24711R
Product Name	EML4 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EML4-ALK fusion protein(EML4)
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27436
Swiss Prot	Q96HE3
Subcellular location	Cytoplasm
Synonyms	EML4; EML-4; C2orf2; Echinoderm microtubule associated protein like 4; Echinoderm microtubule-associated protein-like 4; ELP 120; ELP120; EMAL4_HUMAN; EMAP 4; EMAP-4; EMAP4; EML 4; eml4; Restrictedly overexpressed proliferation associated protein; Restrictedly overexpressed proliferation-associated protein; Ropp 120; ROPP120.
Background	May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic (By similarity).
Application Dilution
IHC-P	ELISA1:500-1000
IHC-F	IHC-P1:200-400
IF