Cytokeratin 8 Polyclonal Antibody

$Lane 1: Human MCF-7 cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HeLa cell lysates probed with Cytokeratin 8 Polyclonal Antibody, Unconjugated (bs-25538R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MCF-7 cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HeLa cell lysates probed with Cytokeratin 8 Polyclonal Antibody, Unconjugated (bs-25538R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bs-25538r
Product Name	Cytokeratin 8 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cytokeratin 8
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3856
Swiss Prot	P05787
Subcellular location	Cytoplasm, Nucleus
Synonyms	card2; Cardiac autoantigen 2 120kD; CK 8; CK8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500