FTO Polyclonal Antibody

$Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human MOLT4 cell lysates probed with FTO Polyclonal Antibody, Unconjugated (bs-25583R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human MOLT4 cell lysates probed with FTO Polyclonal Antibody, Unconjugated (bs-25583R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-25583R
Product Name	FTO Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FTO
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79068
Swiss Prot	Q9C0B1
Subcellular location	Nucleus
Synonyms	Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO_HUMAN; Protein fatso.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500