Phospho-Paxillin (Tyr118) Polyclonal Antibody

$Antigen: bs-3352P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n$

Antigen: bs-3352P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti hospho-Paxillin (Tyr118)Polyclonal Antibody, Unconjugated (bs-3352R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 68kD. Observed band size: 68kD.

Applications

WB
ELISA

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-3352R
Product Name	Phospho-Paxillin (Tyr118) Polyclonal Antibody
Applications	WB, ELISA
Specificity	This phosphorylation site is homologous to Tyr118 in Mouse and Rat.
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr118
Modification Site	Tyr118
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5829
Swiss Prot	P49023
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Paxillin; PXN
Background	Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000