EIF4B Polyclonal Antibody

$Lane 1: Mouse Pancreas tissue lysates; Lane 2: Rat Pancreas tissue lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A431 Cell lysates; Lane 5: Human 293T Cell lysates probed with EIF4B Polyclonal Antibody, Unconjugated (bs-33857R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Pancreas tissue lysates; Lane 2: Rat Pancreas tissue lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A431 Cell lysates; Lane 5: Human 293T Cell lysates probed with EIF4B Polyclonal Antibody, Unconjugated (bs-33857R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-33857r
Product Name	EIF4B Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human EIF4B protein
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1975
Swiss Prot	P23588
Subcellular location	Cytoplasm
Synonyms	EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN.
Background	Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Application Dilution
WB	=1:1000-2000
IHC-P	IHC-P=1:400-800
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500