TCTP Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TCTP Polyclonal Antibody, Unconjugated (bs-3578R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TCTP Polyclonal Antibody, Unconjugated (bs-3578R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit
GuineaPig

Overview
Catalog #	bs-3578R
Product Name	TCTP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TCTP
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7178
Subcellular location	Cytoplasm
Synonyms	FLJ27337; Fortilin; Histamine releasing factor; Histamine-releasing factor; HRF; Immunoglobulin E-dependent; p02; p23; TCTP; TCTP_HUMAN; TPT1; Translationally controlled tumor protein; Translationally-controlled tumor protein; Tumor protein translationally controlled 1; tumor protein translationally-controlled 1; tumor protein, translationally-controlled 1.
Background	TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200