Tuberin Polyclonal Antibody

Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-Tuberin\/TSC2 Polyclonal Antibody, Unconjugated(bs-3586R) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti-Tuberin\/TSC2 Polyclonal Antibody, Unconjugated(bs-3586R) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tuberin Polyclonal Antibody, Unconjugated (bs-3586R) at 1:200 overnight at 4\u00b0C, DAB staining.

NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Tuberin Polyclonal Antibody(bs-3586R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).