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CD4 Polyclonal Antibody

probed with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-4817R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
probed with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-4817R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Thymus Lane 2: Mouse embryo probed with Anti-CD4 Polyclonal Antibody (bs-4817R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Thymus Lane 2: Mouse embryo probed with Anti-CD4 Polyclonal Antibody (bs-4817R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Rat spleen cell lysates; Lane 2: Mouse spleen cell lysates; Lane 3: Rat Lymph node cell lysates; Lane 4: Mouse Lymph node cell lysates ; Lane 5: Rat Thymus cell lysates;Lane 6:Mouse Thymus cell lysates; Lane 7: Rat Small intestine cell lysates; Lane 8: Mouse Small intestine cell lysates; Lane 9: Rat Large intestine cell lysates; Lane 10: Mouse Large intestine cell lysates ; Lane 11: HL60 cell lysates;Lane 12: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-4817R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat spleen cell lysates; Lane 2: Mouse spleen cell lysates; Lane 3: Rat Lymph node cell lysates; Lane 4: Mouse Lymph node cell lysates ; Lane 5: Rat Thymus cell lysates;Lane 6:Mouse Thymus cell lysates; Lane 7: Rat Small intestine cell lysates; Lane 8: Mouse Small intestine cell lysates; Lane 9: Rat Large intestine cell lysates; Lane 10: Mouse Large intestine cell lysates ; Lane 11: HL60 cell lysates;Lane 12: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-4817R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • FCM
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-4817R
Product Name CD4 Polyclonal Antibody
Applications WB, FCM, IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD4
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 920
Swiss Prot P01730
Subcellular location Cell membrane
Synonyms CD4mut; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
Background Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

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