EEF2K Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: EEF2K knockout (KO) HeLa cell lysates probed with EEF2K Polyclonal Antibody, Unconjugated (bs-55060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: 293T cell lysates; Lane 6: Mouse Spleen lysates probed with EEF2K Polyclonal Antibody, Unconjugated (bs-55060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EEF2K) polyclonal Antibody, Unconjugated (bs-55060R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EEF2K) polyclonal Antibody, Unconjugated (bs-55060R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55060R
Product Name	EEF2K Polyclonal Antibody
Applications	WB, IF(ICC), IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	29904
Swiss Prot	O00418
Subcellular location	Cytoplasm
Synonyms	EEF2K;HSU93850;eEF-2K
Background	This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF