Histone H3 (tri methyl K4, serotonyl Q5) Polyclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: lane 1: PTM-1420+H3K4me3\/Q5ser peptides, lane 2: PTM-1420+unmodified peptides, lane 3: PTM-1420, 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: N2a\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: lane 1: PTM-1420+H3K4me3\/Q5ser peptides, lane 2: PTM-1420+unmodified peptides, lane 3: PTM-1420, 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: N2a\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-60081r
Product Name	Histone H3 (tri methyl K4, serotonyl Q5) Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Protein A coupled with the antigen peptide containing H3K4me3/Q5ser.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P68431
Application Dilution
WB	1:300-5000