TCF3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: A549 lysates; Lane 2: Raji lysates probed with TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Human Jurkat cell lysates; Lane 2: Human K562 cell lysates probed with TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human Jurkat cell lysates; Lane 2: Human K562 cell lysates probed with TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-6065r-100ul
Product Name	TCF3 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TCF3/E2A
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6929
Subcellular location	Intracellular
Synonyms	bHLHb21; Class B basic helix-loop-helix protein 21; E12; E2A; E2A immunoglobulin enhancer binding factors E12/E47; E47; Helix loop helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; ITF1; Kappa E2 binding factor; Kappa-E2-binding factor; MGC129647; MGC129648; Negative vitamin D response element binding protein; TCF-3; TCF3; TFE2; TFE2_HUMAN; transcription factor 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor.
Background	Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200