OVCA2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-OVCA2 Polyclonal Antibody, Unconjugated (bs-6298R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-OVCA2 Polyclonal Antibody, Unconjugated (bs-6298R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-6298R
Product Name	OVCA2 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human OVCA2
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm, Nucleus
Synonyms	Candidate tumor suppressor in ovarian cancer 2; Candidate tumor suppressor OVCA2; ovarian cancer associated gene 2 protein; Ovarian cancer gene 2 protein; ovarian tumor suppressor candidate 2; OVCA2_HUMAN.
Background	Ubiquitously expressed protein which belongs to the UPF0483 (OVCA2) family. Encoded in an intron of the gene DPH1/OVCA1 (same strand).
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200