LEU5 Polyclonal Antibody

$Lane 1: Mouse Testis Lysates; Lane 2: Rat Testis Lysates; Lane 3: Rat Lymph Lysates; Lane 4: Human HL-60 cell Lysates; Lane 5: Human SH-SY5Y cell Lysates; Lane 6: Human HeLa cell Lysates. Probed with LEU5 polyclonal Antibody, unconjugated (bs-6395R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis Lysates; Lane 2: Rat Testis Lysates; Lane 3: Rat Lymph Lysates; Lane 4: Human HL-60 cell Lysates; Lane 5: Human SH-SY5Y cell Lysates; Lane 6: Human HeLa cell Lysates. Probed with LEU5 polyclonal Antibody, unconjugated (bs-6395R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-6395R
Product Name	LEU5 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TRIM13/LEU5
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10206
Swiss Prot	O60858
Subcellular location	Cytoplasm, Cell membrane
Synonyms	B cell chronic lymphocytic leukemia tumor suppressor Leu5; CAR; DLEU5; HGNC:9976; LEU5; Leukemia associated protein 5; Putative tumor suppressor RFP2; Ret finger protein 2; RING finger protein 77; RNF77; TRIM13; Tripartite mot protein 13; TRI13_HUMAN.
Background	This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Multiple alternatively spliced transcript variants have been found for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200