Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody

Applications

Reactivity

Mouse
Rat

Overview
Catalog #	bs-70178r
Product Name	Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~52 kDa nAChR_4 protein.
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Fusion protein from the cytoplasmic loop of the _4 subunit of mouse nAChR.
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol.
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Gene ID	108015
Swiss Prot	Q8R493
Synonyms	acetylcholine receptor nicotinic beta 4 (neuronal) antibody, ACHB4_HUMAN antibody, AChR antibody, Cholinergic receptor nicotinic beta 4 antibody, Cholinergic receptor nicotinic beta polypeptide 4 antibody, cholinergic receptor nicotinic beta polypeptide 4 antibody, Chrnb4 antibody, Neuronal acetylcholine receptor subunit beta-4 antibody, Neuronal nicotinic receptor beta 4 subunit antibody
Background	Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits _4_2, whereas _3_4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, _2-containing receptors greatly outnumber receptors that contain _4 (McGehee & Role, 1995; Albuquerque, et al., 2009), and in most brain regions, targeted deletion of the _2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli, et al., 1998) due to the absence of a _ subunit required to form functional nAChRs (Champtiaux & Changeux, 2004).
Application Dilution
WB	1:300-5000