SHOC2 Polyclonal Antibody

$Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse Esophagus lysates; Lane 4: Mouse Large intestine lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Esophagus lysates; Lane 9: Rat Large intestine lysates; Lane 10: Human A549 cell lysates; Lane 11: Human U937 cell lysates\uff1b Lane 12: Human HL-60 cell lysates probed with SHOC2 Polyclonal Antibody, Unconjugated (bs-7934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse Esophagus lysates; Lane 4: Mouse Large intestine lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Esophagus lysates; Lane 9: Rat Large intestine lysates; Lane 10: Human A549 cell lysates; Lane 11: Human U937 cell lysates\uff1b Lane 12: Human HL-60 cell lysates probed with SHOC2 Polyclonal Antibody, Unconjugated (bs-7934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Chicken

Overview
Catalog #	bs-7934r
Product Name	SHOC2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SHOC2/Sur8
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8036
Subcellular location	Cytoplasm, Nucleus
Synonyms	Leucine-rich repeat protein SHOC-2; Leucine-rich repeat protein SHOC-2; Ras-binding protein Sur-8; KIAA0862; Leucine-rich repeat protein SHOC-2; Protein soc-2 homolog; Protein Sur-8 homolog; SHOC2; SHOC2_HUMAN.
Background	Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.Involvement in disease:Defects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) . NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200