ZNRF3 Polyclonal Antibody

$Lane 1: Human HepG2 cell Lysates; Lane 2: Human A549 cell Lysates; Lane 3: Human U-2 OS cell Lysates. Probed with ZNRF3 polyclonal Antibody, unconjugated (bs-9141R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell Lysates; Lane 2: Human A549 cell Lysates; Lane 3: Human U-2 OS cell Lysates. Probed with ZNRF3 polyclonal Antibody, unconjugated (bs-9141R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9141R
Product Name	ZNRF3 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZNRF3
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84133
Swiss Prot	Q9ULT6
Subcellular location	Extracellular
Synonyms	RNF203; BK747E2.3; E3 ubiquitin-protein ligase ZNRF3; RING finger protein 203; Zinc/RING finger protein 3; ZNRF3; KIAA1133
Background	E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
Application Dilution
WB	IHC-P1:200-400
IHC-P	IFIHC-P1:50-200
IHC-F
IF