IGSF9 Polyclonal Antibody

$Antigen: bs-9199P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-9199P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

$Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IGSF9 Polyclonal Antibody, Unconjugated (bs-9199R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining\\n$

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IGSF9 Polyclonal Antibody, Unconjugated (bs-9199R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining\\n

Applications

WB
ELISA
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-9199R
Product Name	IGSF9 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IF(IHC-P)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IGSF9
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	57549
Subcellular location	Extracellular
Synonyms	IGSF9A; Dasm1; Dendrite arborization and synapse maturation protein 1; IGSF9 immunoglobulin superfamily, member 9; Immunoglobulin superfamily member 9A; immunoglobulin superfamily, member 9; Ncaml; Nrt1; Protein turtle homolog A; TUTLA_HUMAN.
Background	Ig (immunoglobulin) superfamily members exhibit functional characteristics including immune responses, growth factor signaling and cell adhesion. IGSF9 (immunoglobulin superfamily, member 9), also known as Nrt1 or IGSF9A, is a 1,179 amino acid single-pass type I membrane protein expressed in a wide variety of fetal tissues at eight and fourteen weeks of gestation. Belonging to the immunoglobulin superfamily and the Turtle family, IGSF9 is thought to play a role in dendrite outgrowth and synapse maturation. IGSF9 contains two fibronectin type-III domains and five Ig-like (immunoglobulin-like) domains. IGSF9 interacts with MAGI-2 and Shank 1, both of which contain SH3 (Src-homology 3) domains. Protein-protein interactions are central events in cellular signal transduction and are often mediated by SH3 domains. IGSF9 is encoded by a gene located on human chromosome 1q23.2 and mouse chromosome 1 H3.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200