RNF170 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.$

Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.

$Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.$

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) and DAB.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Horse
Chicken
Rabbit

Overview
Catalog #	bs-9260R
Product Name	RNF170 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RNF170
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	81790
Synonyms	Putative LAG1 interacting protein; Ring finger protein 170; RN170_HUMAN.
Background	The ring finger is a specialized type of zinc finger of 40 to 60 residues that binds two atoms of zinc and mediates protein-protein interactions. There are five known isoforms of RNF170.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200