HOPX/LAGY Polyclonal Antibody

Human Hela cells probed with HOPX Polyclonal Antibody, A488 conjugated (bs-9462R-A488) (green) at 1:100 for 30 minutes compared to unstained cells (blue) and isotype control (orange).

$Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-LAGY Polyclonal Antibody, Unconjugated (bs-9462R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-LAGY Polyclonal Antibody, Unconjugated (bs-9462R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9462R
Product Name	HOPX/LAGY Polyclonal Antibody
Applications	IHC-P, IHC-F, IF, Flow-Cyt
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HOPX/LAGY
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84525
Swiss Prot	Q9BPY8
Subcellular location	Cytoplasm, Nucleus
Synonyms	HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31; Homeodomain-only protein; Lung cancer-associated Y protein; Not expressed in choriocarcinoma protein 1; Odd homeobox protein 1; HOPX
Background	Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
Application Dilution
IHC-P	WB1:300-5000
IHC-F	IHC-P1:200-400
IF	IHC-P1:50-200
Flow-Cyt