Collagen II Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat cartilage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen II Monoclonal Antibody, Unconjugated (bsm-33382M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat cartilage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen II Monoclonal Antibody, Unconjugated (bsm-33382M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-33382m
Product Name	Collagen II Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human Collagen II
Clonality	Monoclonal
Clone #	3G6
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1280
Swiss Prot	P02458
Subcellular location	Secreted, Extracellular matrix
Synonyms	Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; Collagen alpha-1(II) chain; alpha-1 type II collagen; CO2A1_HUMAN.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF