ZO-1 Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 monoclonal antibody, unconjugated (bsm-41327R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 monoclonal antibody, unconjugated (bsm-41327R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ZO-1\/TJP1 antibody (bsm-41327R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ZO-1\/TJP1 antibody (bsm-41327R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-41327r-100ul
Product Name	ZO-1 Recombinant Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human ZO-1 protein
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7082
Swiss Prot	Q07157
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein;
Application Dilution
WB	1:500-2000
FCM	FCM1μg/Test
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500