AMH Monoclonal Antibody

$Lane 1: Recombinant human AMH protein probed with AMH Monoclonal Antibody, Unconjugated (bsm-43520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human AMH protein probed with AMH Monoclonal Antibody, Unconjugated (bsm-43520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMH) Monoclonal Antibody, Unconjugated (bsm-43520M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMH) Monoclonal Antibody, Unconjugated (bsm-43520M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-43520m
Product Name	AMH Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human AMH protein
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	268
Swiss Prot	P03971
Synonyms	MIS_HUMAN; Anti muellerian hormone; MIF; MIS; Muellerian inhibiting factor; Mullerian inhibiting substance; Muellerian-inhibiting factor; Anti-Muellerian hormone; Muellerian-inhibiting substance.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF