Lactoferrin (9C1) Monoclonal Antibody

$Lane 1: K562 Cell lysates; probed with LTF (119CT80.1.1) Monoclonal Antibody, unconjugated (bsm-51024M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cell lysates; probed with LTF (119CT80.1.1) Monoclonal Antibody, unconjugated (bsm-51024M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells, stained with LTF (119CT80.1.1) Monoclonal Antibody (bsm-51024M) at 1:25 dilution, followed by Goat anti-mouse IgG secondary antibody at 1:200 dilution. Immunofluorescence image shows cytoplasm staining in HeLa cells (green), actin (red), and nuclear counter stain with DAPI (blue).

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-51024M
Product Name	Lactoferrin (9C1) Monoclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This LTF Monoclonal antibody is generated from mouse immunized with LTF recombinant protein.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4057
Swiss Prot	P02788
Synonyms	LF; HLF2; GIG12; HEL110; Lactotransferrin; Lactoferrin; Growth-inhibiting protein 12; Talalactoferrin; LTF
Background	This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis.
Application Dilution
WB	1:300-5000
ELISA	IHC-P1:200-400
IHC-P	IF
IHC-F
IF