RCC1 Monoclonal Antibody

$Lane 1: HL-60 cell lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HL-60 cell lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: HL-60 cell lysates; Lane 2: Hela cell lysates; Lane 3: U-251MG cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Heart tissue lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HL-60 cell lysates; Lane 2: Hela cell lysates; Lane 3: U-251MG cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Heart tissue lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51500m
Product Name	RCC1 Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human RCC1.
Clonality	Monoclonal
Clone #	G14A2
Isotype	IgG1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1104
Swiss Prot	P18754
Subcellular location	Cytoplasm, Nucleus
Synonyms	Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN.
Background	Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Application Dilution
WB	=1:500-2000