ADORA1 Recombinant Antibody

$The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ADORA1 antibody (bsm-54806R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ADORA1 antibody (bsm-54806R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human

Overview
Catalog #	bsm-54806r-100ul
Product Name	ADORA1 Recombinant Antibody
Applications	IHC-P, IHC-F, IF, Flow-Cyt
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human ADORA1
Clonality	Recombinant
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	134
Swiss Prot	P30542
Subcellular location	Cell membrane
Synonyms	RDC7; A1-AR; A1AR; A1R; AA1R; ARA1; Ri; AA1R_BOVIN; ADORA1; AA1R_HUMAN; AA1R_MOUSE; AA1R_RAT;
Application Dilution
IHC-P	=1:100-200
IHC-F	IHC-F=1:100-200
IF	IF=1:50-200
Flow-Cyt	Flow-Cyt=1:50-100