PTPN12 Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: HepG2, 3: NIH\/3T3, 4: C6, \r\n5: Jurkat (low expression)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 88 kDa\r\nObserved MW: 120 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HeLa, 2: HepG2, 3: NIH\/3T3, 4: C6, \r\n5: Jurkat (low expression)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 88 kDa\r\nObserved MW: 120 kDa

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60705r
Product Name	PTPN12 Recombinant Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PTPN12
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5782
Swiss Prot	Q05209
Subcellular location	Cytoplasm
Synonyms	PTN12_HUMAN; Tyrosine-protein phosphatase non-receptor type 12; EC:3.1.3.48; PTP-PEST; Protein-tyrosine phosphatase G1 (PTPG1); protein tyrosine phosphatase non-receptor type 12; PTPG1; PTP-PEST;
Application Dilution
WB	1:300-5000