E cadherin Recombinant Antibody

$4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (E cadherin) monoclonal Antibody, unconjugated (bsm-60971R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (E cadherin) monoclonal Antibody, unconjugated (bsm-60971R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$4% Paraformaldehyde-fixed T-47D (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (E cadherin) monoclonal Antibody, unconjugated (bsm-60971R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed T-47D (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (E cadherin) monoclonal Antibody, unconjugated (bsm-60971R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

The T-47D (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-E cadherin antibody (bsm-60971R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-60971r-100ul
Product Name	E cadherin Recombinant Antibody
Applications	Flow-Cyt, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human E-cadherin
Clonality	Recombinant
Clone #	6E1
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	999
Swiss Prot	P12830
Subcellular location	Cell membrane
Synonyms	Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO; E-cad; L-CAM; Um; Cadherin-1; Uvomorulin; CADH1_BOVIN; CDH1; Epithelial cadherin (E-cadherin); CADH1_HUMAN; CAM 120/80; CADH1_MOUSE; CADH1_RAT;
Application Dilution
Flow-Cyt	=1:100-200
ICC/IF	ICC/IF=1:100-200