PARP2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PARP2 monoclonal antibody, unconjugated (bsm-61895R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PARP2 monoclonal antibody, unconjugated (bsm-61895R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61895r-100ul
Product Name	PARP2 Recombinant Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PARP 2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10038
Swiss Prot	Q9UGN5
Subcellular location	Nucleus
Synonyms	pADPRT-2; hPARP-2; ADPRT2; ADPRTL2.
Background	Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair.Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IF(ICC)	IFICC1:50-200