HOXB4 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with HOXB4 monoclonal antibody, unconjugated (bsm-62255R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with HOXB4 monoclonal antibody, unconjugated (bsm-62255R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IP
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62255r
Product Name	HOXB4 Recombinant Antibody
Applications	WB, IP, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HOX B4
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3214
Swiss Prot	P17483
Subcellular location	Nucleus
Synonyms	HOX2; Hox2.6; Hox2F; hoxb4.
Background	Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I (Pol I) through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element and can activate Pol I promoter escape.
Application Dilution
WB	1:300-5000
IP	1-2ug
ICC/IF	1:50-100