RCHY1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with RCHY1 monoclonal antibody, unconjugated (bsm-62425R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with RCHY1 monoclonal antibody, unconjugated (bsm-62425R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)
IP

Reactivity

Human
Mouse

Overview
Catalog #	bsm-62425r-100ul
Product Name	RCHY1 Recombinant Antibody
Applications	WB, IF(ICC), IP
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RCHY1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25898
Swiss Prot	Q96PM5
Subcellular location	Nucleus
Synonyms	ARNIP; CHIMP; hARNIP; hPirh2; PIRH2E; PIRH2F; RCHY1; RNF199; ZCHY; ZNF363.
Background	E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200
IP	IP1:20-50