MRPL28 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with MRPL28 monoclonal antibody, unconjugated (bsm-62446R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MRPL28 monoclonal antibody, unconjugated (bsm-62446R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MRPL28 antibody (bsm-62446R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MRPL28 antibody (bsm-62446R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MRPL28) monoclonal Antibody, unconjugated (bsm-62446R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MRPL28) monoclonal Antibody, unconjugated (bsm-62446R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
FCM
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62446r-100ul
Product Name	MRPL28 Recombinant Antibody
Applications	WB, FCM, IF(ICC), IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MRPL28
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10573
Swiss Prot	Q13084
Subcellular location	Cytoplasm
Synonyms	L28mt; MAAT1; mrpl28; P15.
Background	Potentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IF(ICC)	IFICC1:50-200
IP	IP1:20-50