phospho-BAD (S75) Recombinant Antibody

$Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-BAD (S75) monoclonal antibody, unconjugated (bsm-63059R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-BAD (S75) monoclonal antibody, unconjugated (bsm-63059R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-63059r
Product Name	phospho-BAD (S75) Recombinant Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser75
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	572
Swiss Prot	Q92934
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Bad (phospho S75); Bad (phospho Ser75); p-Bad (S75);p- Bad (Ser75); p-Bad (phospho Ser75); BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-like protein 8; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; BclXL; Proapoptotic BH3 Only Protein; BAD_HUMAN; Bcl-2-binding component 6.
Application Dilution
WB	1:300-5000