P53(S33) Recombinant Antibody

$4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-P53(S33)) monoclonal Antibody, unconjugated (bsm-63095R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-P53(S33)) monoclonal Antibody, unconjugated (bsm-63095R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-63095r-100ul
Product Name	P53(S33) Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human p53 around the phosphorylation site of S33
Clonality	Recombinant
Clone #	7F5
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7157
Swiss Prot	P04637
Subcellular location	Cytoplasm, Nucleus
Synonyms	TP53 \| p53 (phospho-S33); p-p53; phospho-p53; p-TP53; TP53 \| p53 (phospho-Ser33); BCC7; BMFS5; LFS1; P53; TRP53; Tp53; bbl; bfy; bhy; p44; P53_BOVIN; Tumor suppressor p53; P53_HUMAN; Antigen NY-CO-13; Phosphoprotein p53; P53_MOUSE; P53_RAT;
Application Dilution
WB	=1:1000-2000
ICC/IF	ICC/IF=1:50-200