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Di-Methyl-Histone H3 (K5) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • ChIP
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63100r-100ul
Product Name Di-Methyl-Histone H3 (K5) Monoclonal Antibody
Applications WB, IHC-P, IHC-F, IP, IF, ChIP, Flow-Cyt, ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the methylation site of K5
Clonality Recombinant
Clone # 3F8
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (me2-K5); me2-Histone H3; di-methyl-Histone H3; H3K5me2; me2-H3;
Application Dilution
WB =1:1000-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50
IF IF=1:50-200
ChIP ChIP=1:20-50
Flow-Cyt Flow-Cyt=1:50-100
ICC/IF ICC/IF=1:100-500