EGFR (Y1092) Recombinant Antibody

$4% Paraformaldehyde-fixed A431 (treated with pervanadate (1 mM, 30min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-EGFR (Y1092)) monoclonal Antibody, unconjugated (bsm-63111R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed A431 (treated with pervanadate (1 mM, 30min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-EGFR (Y1092)) monoclonal Antibody, unconjugated (bsm-63111R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-63111r-100ul
Product Name	EGFR (Y1092) Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human EGFR around the phosphorylation site of Y1092
Clonality	Recombinant
Clone #	4B5
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1956
Swiss Prot	P00533
Subcellular location	Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms	EGFR \| ERBB1/HER1 (phospho-Y1092); p-ERBB1/HER1; phospho-ERBB1/HER1; p-EGFR; EGFR \| ERBB1/HER1 (phospho-Tyr1092); ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE;
Application Dilution
WB	=1:1000-2000
IHC-P	IHC-P=1:100-200
IHC-F	IHC-F=1:100-200
IF	IF=1:50-200
ICC/IF	ICC/IF=1:50-200