14-3-3 alpha/beta/zeta/delta Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with 14-3-3 alpha\/beta\/zeta\/delta monoclonal antibody, unconjugated (bsm-63179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with 14-3-3 alpha\/beta\/zeta\/delta monoclonal antibody, unconjugated (bsm-63179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha\/beta\/zeta\/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-63179r-100ul
Product Name	14-3-3 alpha/beta/zeta/delta Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human 14-3-3 alpha/beta
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7529
Swiss Prot	P31946
Subcellular location	Cytoplasm
Synonyms	14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; 14 3 3 zeta; GW128; HS1; KCIP 1; N-terminally processed; YWHAB; YWHAD; YWHAZ.
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500