UBA52 Recombinant Antibody

$The JAR (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-UBA52 antibody (bsm-63309R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The JAR (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-UBA52 antibody (bsm-63309R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-63309r-100ul
Product Name	UBA52 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human UBA52
Clonality	Recombinant
Clone #	10A2
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7311
Subcellular location	Cytoplasm, Nucleus
Synonyms	CEP52; HUBCEP52; L40; RPL40; D8Ertd21e; Gm1863; Rps27a; Ubb; Ubc; RL40_HUMAN; UBA52; Ubiquitin A-52 residue ribosomal protein fusion product 1; UBCEP2; RL40_MOUSE; RL40_BOVIN;
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-200
IHC-F	IHC-F=1:100-200
IF	IF=1:50-200
Flow-Cyt	Flow-Cyt=1:50-100
ICC/IF	ICC/IF=1:50-200