Na+/K+ ATPase _3 (Extracellular) Antibody

Applications

Reactivity

Human

Overview
Catalog #	bsm-70561M
Product Name	Na+/K+ ATPase _3 (Extracellular) Antibody
Applications	WB, IP
Specificity	Clone M025 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase _3 subunit. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the _3 subunit on live cells.
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Clone (M025) was generated from a proprietary antigen related to the native human Na+/K+ ATPase _3 subunit expressed in MeWo melanoma cell line.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG2a
Purification	Purified by Protein G.
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	P54709
Synonyms	Sodium/potassium-transporting ATPase subunit beta-3, ATPB-3, CD298, ATP1B3
Background	The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic _ subunit and a membrane-spanning auxiliary _ subunit. In humans, the Na+/K+ ATPase is a binary complex of an _ subunit that has four isoforms (_1-_4) and a _-subunit that has three isoforms (_1, _2, _3). Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity has anti-cancer effects. The _3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and its increased expression level predicts poor patient outcome. _3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines.
Application Dilution
WB	1:300-5000
IP	1-2ug