| Overview |
| bsm-70605m-100ul |
| Phosphoserine/threonine Antibody |
| WB, IP |
| The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| Phosphoserine/threonine is a mix of Clone psA and clone psB. They were generated from synthetic peptides of phosphoserine and phosphothreonine (coupled to carrier protein), respectively. |
| pSer/Thr |
| Monoclonal |
| psA/psB |
| IgG1 |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| N/A |
| Phosphoser/thr mAb |
| Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
| Application Dilution |
| WB |
1:200-500 |
| IP |
1:50 |
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