RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Western Blot Antibodies



×

Collection Menu
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta-Actin (1A2) Monoclonal Antibody(bsm-33036M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (bsm-33036M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33036m-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33036m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Catalog #1: bsm-33201M-Biotin

Applications: WB

Reactivity: Goat, Cow

Conjugate: Biotin

Catalog #1: bsm-33036m-bf594-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-594

Catalog #1: bsm-4713m-pbs-only-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PBS Only

Catalog #1: bsm-43176m-pbs-only-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PBS Only

Catalog #1: bsm-4713m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PerCP-Cy7

Catalog #1: bsm-4713m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PE-Cy5.5

Catalog #1: bsm-4713m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PerCP

Catalog #1: bsm-4713m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PE-Cy7

Catalog #1: bsm-4713m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: RBITC

Catalog #1: bsm-4713m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: HRP

Catalog #1: bsm-4713m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-4713m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PE-Cy5

Catalog #1: bsm-4713m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: PE

Catalog #1: bsm-4713m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy5.5

Catalog #1: bsm-4713m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: FITC

Catalog #1: bsm-4713m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy7

Catalog #1: bsm-4713m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy5

Catalog #1: bsm-4713m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-750

Catalog #1: bsm-4713m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Biotin

Catalog #1: bsm-4713m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy3

Catalog #1: bsm-4713m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-647

Catalog #1: bsm-4713m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-594

Catalog #1: bsm-4713m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-405

Catalog #1: bsm-4713m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-680

Catalog #1: bsm-4713m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-350

Catalog #1: bsm-4713m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-488

Catalog #1: bsm-4713m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: AbBy Fluor-555

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-4713m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-4713m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: APC-Cy7

Catalog #1: bsm-4713m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: APC-Cy5.5

Catalog #1: bsm-4713m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: APC

Catalog #1: bsm-33036m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PerCP-Cy7

25 ug total protein per lane of various lysates (see on figure) probed with SIRT2 monoclonal antibody, unconjugated (bsm-52221R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT2 Monoclonal Antibody, Unconjugated (bsm-52221R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT2 Monoclonal Antibody, Unconjugated (bsm-52221R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52221r-TR

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-43176m-pe-cy7-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PE-Cy7

Catalog #1: bsm-43176m-percp-Cy5.5-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43176m-fitc-100ul

Applications: WB

Reactivity: Bovine

Conjugate: FITC

Catalog #1: bsm-43176m-cy7-100ul

Applications: WB

Reactivity: Bovine

Conjugate: Cy7

Catalog #1: bsm-43176m-percp-cy7-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PerCP-Cy7

Catalog #1: bsm-43176m-Cy5.5-100ul

Applications: WB

Reactivity: Bovine

Conjugate: Cy5.5

Catalog #1: bsm-43176m-biotin-100ul

Applications: WB

Reactivity: Bovine

Conjugate: Biotin

Catalog #1: bsm-43176m-rbitc-100ul

Applications: WB

Reactivity: Bovine

Conjugate: RBITC

Catalog #1: bsm-43176m-cy5-100ul

Applications: WB

Reactivity: Bovine

Conjugate: Cy5

Catalog #1: bsm-43176m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PE-Cy5.5

Catalog #1: bsm-43176m-pe-cy5-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PE-Cy5

Catalog #1: bsm-43176m-bf647-100ul

Applications: WB

Reactivity: Bovine

Conjugate: AbBy Fluor-647

Catalog #1: bsm-43176m-percp-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PerCP