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Western Blot Antibodies



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Catalog #1: bs-1557g-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human tumor tissue labeled with Anti-HPV16-E6 Polyclonal Antibody, Unconjugated(bs-0990R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HPV16 E6 protein Polyclonal Antibody, Unconjugated (bs-0990R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with XXX Antibody(bs-R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange). Antibody(bs-0990R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0990R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: Unconjugated

Antigen: bs-10446P, 0.2ug\/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; TMB staining; Read the data in MicroplateReader by 450nm.
Hela (Positive) and RSC96 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha HPV16-E7Antibody (bs-10446R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
Lane 1: Recombinant HPV16 E7 protein Bacterial lysate, DsbC & His(bs-49101L) probed with HPV16 E7 Polyclonal Antibody, Unconjugated (bs-10446R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10446R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-BRLF1 Polyclonal Antibody, Unconjugated (bs-4542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-4542R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Virus

Conjugate: Unconjugated

Transfected HeLa cell lysates probed with Anti-CSFV Envelope glycoprotein E2 Polyclonal Antibody, Unconjugated (bs-4527R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: Recombinant CSFV E2 protein (200ng) (bs-20116P). Probed with CSFV E2 polyclonal Antibody, unconjugated (bs-4527R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4527R

Applications: WB, ELISA

Reactivity: CSFV

Conjugate: Unconjugated

Catalog #1: bs-0271r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4507r

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0221R

Applications: WB

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-1557G-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus

Conjugate: Biotin

Catalog #1: bs-1557G-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: FITC

Catalog #1: bs-10446R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus, Others

Conjugate: Biotin

Catalog #1: bs-10446R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus, Others

Conjugate: HRP

Catalog #1: bs-0271R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-10446R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: AbBy Fluor-647

Catalog #1: bs-4542R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus

Conjugate: Biotin

Catalog #1: bs-10446R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: FITC

Catalog #1: bs-0990R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: FITC

Catalog #1: bs-10446R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: APC

Catalog #1: bs-10446R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: AbBy Fluor-594

Catalog #1: bs-10446R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: AbBy Fluor-488

Catalog #1: bs-0271R-PE

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-10446R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: PE

Catalog #1: bs-4527R-Biotin

Applications: WB, ELISA

Reactivity: CSFV

Conjugate: Biotin

Catalog #1: bs-4542R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus

Conjugate: HRP

Catalog #1: bs-1557G-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Virus

Conjugate: HRP

Catalog #1: bs-1557G-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: Cy3

Catalog #1: bs-0271R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-1557g-percp-cy7-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: PerCP-Cy7

Catalog #1: bs-4507R-PE

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PE

Catalog #1: bs-4507R-PE-Cy7

Applications: WB

Reactivity:

Conjugate: PE-Cy7

Catalog #1: bs-4507R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bs-4507R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC

Catalog #1: bs-4507R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bs-4507R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-594

Catalog #1: bs-4507R-PE-Cy5

Applications: WB

Reactivity:

Conjugate: PE-Cy5

Catalog #1: bs-4507R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-4507R-PE-Cy5.5

Applications: WB

Reactivity:

Conjugate: PE-Cy5.5

Catalog #1: bs-4507R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP

Catalog #1: bs-4507R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-4507R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-4507R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bs-4507R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: RBITC

Catalog #1: bs-4507R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-555

Catalog #1: bs-4507R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-350

Catalog #1: bs-4507R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-750

Catalog #1: bs-4507R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-647

Catalog #1: bs-4507R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5.5

Catalog #1: bs-4507R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-680