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Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-6313P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6313r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-0081R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0081r-100ul

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.

Catalog #1: bs-0756R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
RSC96 probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1970R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated (bs-3195R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3195R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 12kD. Observed band size:48kD.\\n
Image provided by the Independent Validation Program (badge number 29759). Lane 1: HeLa cell extract, Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0052R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Human 293T cells probed with CD32B(Tyr292) Polyclonal Antibody, unconjugated (bs-6031R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD32B(Tyr292) Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6031R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TBK1 (Ser172) Polyclonal Antibody, Unconjugated(bs-3440R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Raji cell lysates; Lane 2: Human HeLa cell lysates probed with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (bs-3440R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3440R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4507r

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Antigen: bs-5490P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Mouse large intestine lysates probed with Ron (Tyr1238) Polyclonal Antibody, unconjugated (bs-5490R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-5490R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PSD-95 Polyclonal Antibody (bs-0179R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human SH-SY5Y cell lysates probed with PSD95 Polyclonal Antibody, Unconjugated (bs-0179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0179R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Fas Polyclonal Antibody, Unconjugated (bs-0215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus tissue lysates; Lane 2: Rat Thymus tissue lysates; Lane 3: Human HepG2 cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human HT1080 cell Lysates; Lane 6: Human A431 cell Lysates probed with CD95\/FAS Polyclonal Antibody, Unconjugated (bs-0215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2993R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-LIN9 Polyclonal Antibody, Unconjugated (bs-18283R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n
Mouse bone lysates probed with Anti-LIN9 Polyclonal Antibody, Unconjugated (bs-18283R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:10000 for 90 min at 37˚C.\\n
25 ug total protein per lane of various lysates (see on figure) probed with LIN9 polyclonal antibody, unconjugated (bs-18283R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-18283r-TR

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney cancer labeled with Anti-HIF-2\u03b1 Polyclonal Antibody, Unconjugated (bs-1447R) followed by conjugation to the secondary antibody and DAB staining
Rat lung lysates probed with Rabbit Anti-HIF2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 and Mouse Anti-GAPDH (bsm-0978M) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
HepG2 cells(black) were fixed with 70% ice-cold methanol overnight at 4\u2103,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF2 alpha Polyclonal Antibody(bs-1447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1447R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1299r

Applications: WB, ELISA, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3195R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

L1 mouse liver lysates, L2 mouse brain lysates probed with Anti FADD Polyclonal Antibody, Unconjugated (bs-0511R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 23kD
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with FADD Polyclonal Antibody (bs-0511R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with FADD Polyclonal Antibody (bs-0511R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-0511R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0336R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Monkey

Conjugate: HRP

Catalog #1: bs-3398R-PE

Applications: WB, FCM

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-3398r

Applications: WB, ELISA, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (bs-8204R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (bs-8204R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-8204R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0335R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Monkey

Conjugate: HRP

Catalog #1: bs-6313R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bs-6031R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Monkey

Conjugate: Biotin

Catalog #1: bs-0756R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-6313R-BF488

Applications: WB, IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor-488

Catalog #1: bs-6313R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-6031R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: FITC

Catalog #1: bs-3195R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0756R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-6313R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Others

Conjugate: Cy3

Mouse bone lysates probed with Rabbit Anti-CLN3 Polyclonal Antibody, Unconjugated (bs-8016R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Mouse heart lysates probed with CLN3 Polyclonal Antibody, Unconjugated (bs-8016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8016r

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-6313P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0920r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0756R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-0756R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-0756R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-1970R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-6313R-BF594

Applications: WB, IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor-594

Catalog #1: bs-3195R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAPDH Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52262r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6313R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-1970R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1970R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-1299R-Cy3

Applications: WB, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0756R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0052R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0336R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Monkey

Conjugate: Unconjugated